Table of Contents
What is the EAE model?
Inflammatory EAE Model of Multiple Sclerosis Experimental autoimmune encephalomyelitis (EAE) is an animal model of multiple sclerosis. This model is produced by administering a myelin basic protein peptide (MBP) fragment that induces an autoimmune response directed to the myelin sheath surrounding motor neurons.
What are EAE mice?
EAE by Active Immunization In the active immunization method, the mouse is immunized using neuro antigens, such as myelin antigens or spinal cord homogenate, which causes development of activated myelin-specific T cells, trafficking of immune cells into the CNS, and direct damage and inflammation in the CNS.
Is EAE a good model for MS?
Many elements of this cascade of events have been identified, tested or confirmed in EAE. From the pathogenesis point of view, therefore, EAE is a good model for studying MS mechanisms, even more so than for testing or developing drugs (Farooqi et al., 2010).
What is mog35 55 peptide?
MOG (35-55) or myelin oligodendrocyte glycoprotein (MOG) 35-55 is a minor component of CNS myelin. MOG (35-55) produces a relapsing-remitting neurological disease with extensive plaque-like demyelination, common to the manifestations of multiple sclerosis.
How do you score EAE?
These defects are quantified using a standard EAE scoring system on a 0–5 disease severity scale: 0, no disease; 1, loss of tail tone; 2, hind limb weakness; 3, hind limb paralysis; 4, hind limb paralysis and forelimb paralysis or weakness; and 5, moribund/death10.
How do you induce EAE?
EAE is induced in C57BL/6 mice1 by immunization with an emulsion of MOG35-55 or MOG1-125 in complete Freund’s adjuvant (CFA), followed by administration of pertussis toxin in PBS, first on the day of immunization and then again the following day.
How is EAE induced?
Actively induced EAE consists of an induction phase and an effector phase. The induction phase of the disease involves the priming of myelin epitope–specific CD4+ T cells following immunization with myelin proteins or peptides in complete Freund’s adjuvant (CFA).
What is Cuprizone used for?
Cuprizone is used to produce toxic demyelination that resembles the demyelination that occurs in MS (Torkildsen et al., 2008).
What is Cuprizone model?
The cuprizone model is a model for toxic demyelination. In this model, young mice are fed with the copper chelator cuprizone, leading to oligodendrocyte death and a subsequent reversible demyelination. Spontaneous remyelination can be seen as early as 4 days after withdrawal of cuprizone.
How does Cuprizone cause demyelination?
Oral intoxication with the copper-chelator cuprizone induces oligodendrocyte apoptosis within a few days, which is closely followed by the activation of the innate immune cells in the brain, i.e., astrocytes and microglia, finally leading to demyelination of distinct white and grey matter brain areas.
Can autoimmune encephalitis be cured?
“Autoimmune encephalitis is a treatable disease. But some cases end up being very complex,” Dr. Day says. “We see patients who have spent an inordinate amount of time in the hospital and even the intensive care unit due to the many complications of this disease.”
Can you cure autoimmune encephalitis?
What kind of doctor treats autoimmune encephalitis?
Multidisciplinary Treatment of Autoimmune Encephalitis These include oncologists, urologists, physical and occupational therapists, epileptologists, sleep specialties and specialists in autonomic nervous system disorders.
What is the Mog-induced EAE model?
The myelin oligodendrocyte glycoprotein (MOG)-induced EAE model is ideal for exploring this immune-mediated mechanism of neuroinflammation and demyelination. Our MOG-induced EAE model is well-characterized and commonly used in the development of a variety of MS therapeutics.
What is the difference between antigen induced EAE and Mog-induced EAE?
The disease induced by either antigen is similar, except that EAE induced by whole MOG protein is reported to be dependent on B cells; this may be of interest if your compound targets B cells.
What is the best Mog antigen to use for EAE?
Most often MOG 35-55 peptide antigen is used, but the model can also be run with whole MOG protein (MOG 1-125 ). The disease induced by either antigen is similar, except that EAE induced by whole MOG protein is reported to be dependent on B cells; this may be of interest if your compound targets B cells.
Is mog35-55peptide the best model for EAE?
Immunization of C57BL/6 mice with MOG35-55peptide is one of the most widely distributed EAE models and can be considered as a reliable, replicable and well-to-use animal model. In many neuroimmunological laboratories, MOG35-55induced EAE is established as the model of choice while other EAE models are used for more specific experimental questions.